Helicobacter Pylori antibody (AA 323-445)

Catalog No. ABIN7477495

Product Details anti-Helicobacter Pylori Antibody

Target: Helicobacter Pylori (H. pylori)

Binding Specificity: AA 323-445

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Helicobacter Pylori antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant Helicobacter pylori Catalase protein fragment (around aa323-445) (Exact sequence is proprietary)

Clone: MSVA-466M

Isotype: IgG2b, kappa

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Gastric tissue with known helicobacter pylori infection should show a distinct staining of bacteria on the surface and/or within glands.

Negative Control: Gastric tissue: Normal epithelial and stromal cells should not stain.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Helicobacter Pylori

Target: Helicobacter Pylori (H. pylori)

Alternative Name: Helicobacter Pylori (H. pylori Products)

Target Type: Virus

Background: H. pylori,Helicobacter Pylori antibody validated for immunohistochemistry on 76 different Normal Tissues