Hepatocyte Specific Antigen antibody

Catalog No. ABIN7477487

Product Details anti-Hepatocyte Specific Antigen Antibody

Target: Hepatocyte Specific Antigen (HepPar-1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hepatocyte Specific Antigen antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Extract of a formalin-fixed, rejected-allograft of a human liver

Clone: MSVA-OCE5

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Liver: Virtually all hepatocytes should show an at least moderate granular cytoplasmic staining.

Negative Control: Tonsil: No staining should be seen.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Hepatocyte Specific Antigen

Target: Hepatocyte Specific Antigen (HepPar-1)

Alternative Name: Hepatocyte Specific (HepPar-1 Products)

Background: Not Known,Hepatocyte Specific antibody validated for immunohistochemistry on 76 different Normal Tissues