GP2 antibody (AA 35-179)
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- Target See all GP2 products
- GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))
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Binding Specificity
- AA 35-179
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GP2 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Immunogen
- Recombinant fragment of human GP2 protein (around aa 35-179) (exact sequence is proprietary)
- Clone
- MSVA-475M
- Isotype
- IgG
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- Application Notes
- IHC 1:100-1:200
- Comment
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Positive Control: Pancreas: Acinar cells should show a strong immunostaining with apical predominance. Duodenum: Glandular cells of Brunner glands should show a weak to moderate GP2 positivity.
Negative Control: Colon: GP2 staining should be completely absent in glandular and stromal cells.
- Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Target
- GP2 (Glycoprotein 2 (Zymogen Granule Membrane) (GP2))
- Alternative Name
- GP2 (GP2 Products)
- Synonyms
- ZAP75 antibody, 2310037I18Rik antibody, AV060639 antibody, gp80 antibody, glycoprotein 2 antibody, glycoprotein 2 (zymogen granule membrane) antibody, GP2 antibody, Gp2 antibody
- Target Type
- Viral Protein
- Background
- Glycoprotein 2 (zymogen granule membrane), GP2, Pancreatic zymogen granule membrane associated protein GP2, Pancreatic zymogen granule membrane protein GP-2, ZAP75,GP2 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P55259
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