Recombinant Elastin antibody

Catalog No. ABIN7477467

Product Details anti-Elastin Antibody

Target: Elastin (ELN)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Elastin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant full-length human Elastin protein

Clone: MSVA-648R

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A moderate to strong elastin immunostaining should be seen of fibres in the lamina propria, around smooth muscle cells of the muscularis mucosae, and in blood vessels (subendothelial).

Negative Control: Colon: Elastin immunostaining should be absent in all epithelial cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Elastin

Target: Elastin (ELN)

Alternative Name: Elastin (ELN Products)

Background: Elastin, ELN, SVAS, Tropoelastin, WBS, WS,Elastin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P15502

Pathways: Regulation of Actin Filament Polymerization