Recombinant Desmin antibody

Catalog No. ABIN7477457

Product Details anti-Desmin Antibody

Target: Desmin (DES)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant full-length human desmin protein

Clone: MSVA-651M

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Appendix: Smooth muscle cells in the lamina muscularis mucosae and muscularis propria must show a strong cytoplasmic staining while staining should be at least moderate in most smooth muscle cells of blood vessels and in dispersed myofibroblasts.

Negative Control: Appendix: Desmin staining should not be seen in lymphocytes and epithelial cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: Desmin (DES Products)

Background: CMD1I, CSM1, CSM2, DES, Intermediate filament protein,Desmin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P17661