Recombinant Decorin antibody (AA 212-336)

Catalog No. ABIN7477455

Product Details anti-Decorin Antibody

Target: Decorin (DCN)

Antibody Type: Recombinant Antibody

Binding Specificity: AA 212-336

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Decorin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant human Decorin protein fragment (around aa212-336) (exact sequence is proprietary)

Clone: MSVA-537R

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Fallopian tube: A strong staining should be seen in the stroma while epithelial cells remain negative.

Negative Control: Fallopian tube: Epithelial cells should not show any staining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Decorin

Target: Decorin (DCN)

Alternative Name: Decorin (DCN Products)

Background: Bone proteoglycan II, CSCD, Dermatan sulphate proteoglycans II (DSPG2), PG40, PGII, PGS2, Proteoglycan core protein, SLRR1B, Small leucine rich protein 1B,Decorin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P07585

Pathways: Glycosaminoglycan Metabolic Process