KRT15 antibody

Catalog No. ABIN7477445

Product Details anti-KRT15 Antibody

Target: KRT15 (Keratin 15 (KRT15))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT15 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant full-length human KRT15 protein

Clone: MSVA-615M

Isotype: IgG

Application Details

Application Notes: IHC 1:50-1:100

Comment:

Positive Control: Tonsil: All cells of the surface squamous epithelium and most squamous cells of the crypts should show strong KRT15 staining.

Negative Control: Tonsil: All lymphocytes and blood vessels must not show any KRT15 staining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for KRT15

Target: KRT15 (Keratin 15 (KRT15))

Alternative Name: Cytokeratin 15 (KRT15 Products)

Background: CK15, Cytokeratin 15, K1CO, Ka15, Keratin 15 basic, Keratin 15 beta, Keratin complex 1 acidic gene 15, Keratin type I cytoskeletal 15, KRT15, KRTB, KRTL15, Type I cytoskeletal 15, Type I keratin Ka15,Cytokeratin 15 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P19012