Recombinant Keratin 10 antibody

Catalog No. ABIN7477439

Product Details anti-Keratin 10 Antibody

Target: Keratin 10 (KRT10)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant full-lenth human KRT10 protein.

Clone: MSVA-610M

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Skin: All squamous epithelial cells (except the basal cell layer) should show strong cytoplasmic staining, while the basal cell layer remains KRT10 negative or shows only minimal staining.

Negative Control: Colon: All epithelial, inflammatory, stromal and muscle cells must not show any KRT10 staining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: Cytokeratin 10 (KRT10 Products)

Background: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10,Cytokeratin 10 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P13645