Recombinant Keratin 10 antibody
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- Target See all Keratin 10 (KRT10) Antibodies
- Keratin 10 (KRT10)
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Keratin 10 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Immunogen
- Recombinant full-lenth human KRT10 protein.
- Clone
- MSVA-610M
- Isotype
- IgG
- Top Product
- Discover our top product KRT10 Primary Antibody
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- Application Notes
- IHC 1:100-1:200
- Comment
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Positive Control: Skin: All squamous epithelial cells (except the basal cell layer) should show strong cytoplasmic staining, while the basal cell layer remains KRT10 negative or shows only minimal staining.
Negative Control: Colon: All epithelial, inflammatory, stromal and muscle cells must not show any KRT10 staining.
- Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Target
- Keratin 10 (KRT10)
- Alternative Name
- Cytokeratin 10 (KRT10 Products)
- Synonyms
- BCIE antibody, BIE antibody, CK10 antibody, EHK antibody, K10 antibody, KPP antibody, D130054E02Rik antibody, K1C1 antibody, Krt-1.10 antibody, Krt1-10 antibody, Ka10 antibody, Ker10 antibody, KRT10 antibody, KRT25 antibody, KRT27 antibody, KRT28 antibody, keratin 10 antibody, keratin 10, type I S homeolog antibody, keratin, type I cytoskeletal 25 antibody, KRT10 antibody, Krt10 antibody, krt10.S antibody, LOC101085587 antibody
- Background
- BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10,Cytokeratin 10 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P13645
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