Recombinant CTLA4 antibody
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- Target See all CTLA4 Antibodies
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This CTLA4 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Clone
- MSVA-152R
- Isotype
- IgG
- Top Product
- Discover our top product CTLA4 Primary Antibody
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- Application Notes
- IHC 1:50-1:100
- Comment
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Positive Control: Tonsil: A moderate strong membranous and cytoplasmic CTLA4 immunostaining should be seen in a subset of T-lymphocytes with strongest staining in follicular T-helper cells of germinal centres.
Negative Control: Tonsil: The majority of lymphocytes in germinal centres and all epithelial cells should stain negative.
- Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Target
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
- Alternative Name
- CTLA-4 (CTLA4 Products)
- Synonyms
- CTLA-4 antibody, CTLA4 antibody, CD152 antibody, Cd152 antibody, Ctla-4 antibody, Ly-56 antibody, CD antibody, CELIAC3 antibody, GRD4 antibody, GSE antibody, IDDM12 antibody, sCTLA4 antibody, cytotoxic T-lymphocyte associated protein 4 antibody, cytotoxic T-lymphocyte-associated protein 4 antibody, CTLA4 antibody, Ctla4 antibody
- Background
- ALPS5, CD152, Celiac disease 3 (CELIAC3), Cytotoxic T-lymphocyte-associated antigen 4 (CTLA4), GRD4, GSE, ICOS, Insulin-dependent Diabetes Mellitus 12 (IDDM12),CTLA-4 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P16410
- Pathways
- Cancer Immune Checkpoints
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