Recombinant Elastase 3B antibody (AA 82-238)

Catalog No. ABIN7477421

Product Details anti-Elastase 3B Antibody

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Antibody Type: Recombinant Antibody

Binding Specificity: AA 82-238

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Elastase 3B antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)

Clone: MSVA-410M

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Pancreas: Acinar cells should show a strong CELA3B immunostaining with apical predominance.

Negative Control: Kidney: CELA3B staining should be absent in all epithelial cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Alternative Name: CELA3B (ELA3B Products)

Background: Chymotrypsin like elastase family member 3B (CELA3B), ELA3B, Elastase IIIB, Protease E,CELA3B antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P08861