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CD27 antibody (AA 28-170)

CD27 Reactivity: Human IHC Host: Mouse Monoclonal MSVA-027M unconjugated
Catalog No. ABIN7477407
  • Target See all CD27 Antibodies
    CD27 (CD27 Molecule (CD27))
    Binding Specificity
    • 16
    • 15
    • 9
    • 8
    • 8
    • 6
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 28-170
    Reactivity
    • 174
    • 62
    • 43
    • 5
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 110
    • 73
    • 22
    • 3
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 115
    • 95
    • 2
    Monoclonal
    Conjugate
    • 74
    • 21
    • 20
    • 14
    • 10
    • 7
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD27 antibody is un-conjugated
    Application
    • 135
    • 76
    • 50
    • 32
    • 29
    • 17
    • 16
    • 14
    • 13
    • 13
    • 10
    • 8
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC)
    Immunogen
    Recombinant human CD27 protein fragment (aa 28-170) (exact sequence is proprietary)
    Clone
    MSVA-027M
    Isotype
    IgG
    Top Product
    Discover our top product CD27 Primary Antibody
  • Application Notes
    IHC 1:100-1:200
    Comment

    Positive Control: Tonsil: A strong, membranous staining should be seen in the majority of interfollicular lymphocytes but also in a fraction of lymphocytes in the germinal centres and the mantle zone.

    Negative Control: Tonsil: CD27 staining should be absent in all epithelial cells and a fraction of lymphocytes.

    Protocol
    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • Target
    CD27 (CD27 Molecule (CD27))
    Alternative Name
    CD27 (CD27 Products)
    Synonyms
    TNFRSF7 antibody, S152 antibody, Tnfrsf7 antibody, Tp55 antibody, S152. LPFS2 antibody, T14 antibody, CD27 molecule antibody, CD27 antigen antibody, CD27 antibody, Cd27 antibody
    Background
    LPFS2, S152, T cell activation antigen S152, T-cell activation antigen CD27, T14, TNFRSF7, TNFSF7, Tp55, Tumor necrosis factor receptor superfamily member 7,CD27 antibody validated for immunohistochemistry on 76 different Normal Tissues
    UniProt
    P26842
    Pathways
    Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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