CD27 antibody (AA 28-170)
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- Target See all CD27 Antibodies
- CD27 (CD27 Molecule (CD27))
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Binding Specificity
- AA 28-170
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD27 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Immunogen
- Recombinant human CD27 protein fragment (aa 28-170) (exact sequence is proprietary)
- Clone
- MSVA-027M
- Isotype
- IgG
- Top Product
- Discover our top product CD27 Primary Antibody
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- Application Notes
- IHC 1:100-1:200
- Comment
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Positive Control: Tonsil: A strong, membranous staining should be seen in the majority of interfollicular lymphocytes but also in a fraction of lymphocytes in the germinal centres and the mantle zone.
Negative Control: Tonsil: CD27 staining should be absent in all epithelial cells and a fraction of lymphocytes.
- Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Target
- CD27 (CD27 Molecule (CD27))
- Alternative Name
- CD27 (CD27 Products)
- Synonyms
- TNFRSF7 antibody, S152 antibody, Tnfrsf7 antibody, Tp55 antibody, S152. LPFS2 antibody, T14 antibody, CD27 molecule antibody, CD27 antigen antibody, CD27 antibody, Cd27 antibody
- Background
- LPFS2, S152, T cell activation antigen S152, T-cell activation antigen CD27, T14, TNFRSF7, TNFSF7, Tp55, Tumor necrosis factor receptor superfamily member 7,CD27 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P26842
- Pathways
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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