Recombinant CD22 antibody (AA 52-178)
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- Target See all CD22 Antibodies
- CD22 (CD22 Molecule (CD22))
- Antibody Type
- Recombinant Antibody
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Binding Specificity
- AA 52-178
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This CD22 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Immunogen
- Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)
- Clone
- MSVA-022R
- Isotype
- IgG
- Top Product
- Discover our top product CD22 Primary Antibody
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anti-CD22 Molecule (CD22) (AA 621-725) antibody
CD22 Reactivity: Human FACS, WB, ELISA, IHC, ICC Host: Mouse Monoclonal 2H1C4 unconjugated
anti-CD22 Molecule (CD22) (AA 621-725) antibodyCD22 Reactivity: Human FACS, WB, ELISA, ICC Host: Mouse Monoclonal 1A3A11 unconjugated
anti-CD22 Molecule (CD22) (AA 52-178) antibodyCD22 Reactivity: Human FACS, WB, ELISA, IHC, IF, Coat, StM Host: Mouse Monoclonal BLCAM-1795 unconjugated
anti-CD22 Molecule (CD22) (AA 52-178) antibodyCD22 Reactivity: Human FACS, WB, ELISA, IHC, IF, Coat Host: Mouse Monoclonal BLCAM-1796 unconjugated
anti-CD22 Molecule (CD22) antibodyCD22 Reactivity: Human, Non-Human Primate FACS, WB, IP Host: Mouse Monoclonal MEM-01 unconjugated
anti-CD22 Molecule (CD22) antibody (PE)CD22 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal MEM-01 PE
anti-CD22 Molecule (CD22) antibody (FITC)CD22 Reactivity: Human FACS Host: Mouse Monoclonal IS7 FITC
anti-CD22 Molecule (CD22) (AA 20-687) antibodyCD22 Reactivity: Human FACS, WB, ELISA, IF, IP Host: Rabbit Monoclonal DM13 unconjugated
anti-CD22 Molecule (CD22) antibodyCD22 Reactivity: Human, Mouse, Rat, Monkey, Dog WB, IHC, IHC (p) Host: Mouse Monoclonal 4C3 unconjugated
anti-CD22 Molecule (CD22) (AA 708-847) antibodyCD22 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
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- Application Notes
- IHC 1:100-1:200
- Comment
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Positive Control: Tonsil or appendix: A strong, predominantly membranous CD22 staining of the germinal centre and mantle zone B-cells as well as of few interfollicular B-cells should be seen.
Negative Control: Tonsil or appendix: A large fraction of the interfollicular lymphocytes and all epithelial cells must not show any CD22 staining.
- Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Target
- CD22 (CD22 Molecule (CD22))
- Alternative Name
- CD22 (CD22 Products)
- Synonyms
- A530093D23 antibody, Lyb-8 antibody, Lyb8 antibody, SIGLEC-2 antibody, SIGLEC2 antibody, siglec-2 antibody, CD22 antigen antibody, CD22 molecule antibody, Cd22 antibody, CD22 antibody
- Background
- B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14,CD22 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P20273
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