Recombinant CD5 antibody (AA 32-372)

Catalog No. ABIN7477395

Product Details anti-CD5 Antibody

Target: CD5

Antibody Type: Recombinant Antibody

Binding Specificity: AA 32-372

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant fragment (around aa32-372) corresponding to human CD5 protein (extracellular).

Clone: MSVA-005R

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: A strong CD5 staining of virtually all T-cells in both T-zones and in germinal centres as well as an at least weak to moderate staining of dispersed B-cells in the mantle zone should be seen.

Negative Control: Tonsil: CD5 staining should be absent in germinal centre B-cells and in surface epithelial cells (A fraction of crypt epithelial cells may stain positive).

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for CD5

Target: CD5

Alternative Name: CD5 (CD5 Products)

Background: CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5,CD5 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P06127