Recombinant Cadherin-16 antibody

Catalog No. ABIN7477379

Product Details anti-Cadherin-16 Antibody

Target: Cadherin-16 (CDH16)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cadherin-16 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Recombinant human full-length CDH16 protein

Clone: MSVA-516R

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Kidney: A strong staining should be seen in distal tubuli and collecting ducts while the staining is at least moderate in proximal tubuli.

Negative Control: Colon: All cells should be CDH16 negative.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Cadherin-16

Target: Cadherin-16 (CDH16)

Alternative Name: Cadherin 16 / CDH16 (CDH16 Products)

Synonyms: cadherin 16 antibody, CDH16 antibody, Cdh16 antibody

Background: Cadherin-16 (CDH16), Kidney-specific cadherin, Ksp-cadherin antibody,Cadherin 16 / CDH16 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: O75309