Mafg antibody

Catalog No. ABIN7469480

Product Details anti-Mafg Antibody

Target: Mafg (Transcription Factor MafG (Mafg))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mafg antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ChIP DNA-Sequencing (ChIP-seq), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MAFG. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse muscle , rat muscle Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.53 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for Mafg

Target: Mafg (Transcription Factor MafG (Mafg))

Alternative Name: MAFG (Mafg Products)

Synonyms: zgc:85896 antibody, MAFG antibody, hMAF antibody, AA545192 antibody, C630022N07Rik antibody, v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog Gb antibody, MAF bZIP transcription factor G antibody, v-maf musculoaponeurotic fibrosarcoma oncogene family, protein G (avian) antibody, mafgb antibody, MAFG antibody, Mafg antibody

Background:

Synonyms: MAF bZIP transcription factor G , hMAF

Background: Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877, MafG, or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM]

Molecular Weight: 18 kDa

Gene ID: 4097

UniProt: O15525