POLE3 antibody
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- Target See all POLE3 Antibodies
- POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLE3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- Cross-Reactivity
- Human, Mouse
- Purification
- Purified by antigen-affinity chromatography.
- Grade
- KO Validated
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human DNA polymerase epsilon 3. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product POLE3 Primary Antibody
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- Application Notes
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: 293T Validation: KO/KD
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.01 mg/mL
- Buffer
- 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))
- Alternative Name
- DNA polymerase epsilon 3, accessory subunit (POLE3 Products)
- Synonyms
- NCU04548.1 antibody, GB14966 antibody, zgc:73351 antibody, 1810034K18Rik antibody, YBL1 antibody, CHARAC17 antibody, CHRAC17 antibody, p17 antibody, DNA polymerase epsilon 3, accessory subunit antibody, polymerase (DNA directed), epsilon 3, accessory subunit antibody, DNA polymerase epsilon antibody, DNA polymerase epsilon p17 subunit antibody, DNA polymerase epsilon subunit 3 antibody, polymerase (DNA directed), epsilon 3 (p17 subunit) antibody, POLE3 antibody, pole3 antibody, NCU04548 antibody, CpipJ_CPIJ008666 antibody, Bm1_04865 antibody, Bm1_07285 antibody, CTRG_00708 antibody, LOAG_09571 antibody, LOC726923 antibody, Pole3 antibody
- Background
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Synonyms: DNA polymerase epsilon 3, accessory subunit , CHARAC17 , CHRAC17 , CHRAC2 , YBL1 , p17
Background: POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM]
- Molecular Weight
- 17 kDa
- Gene ID
- 54107
- UniProt
- Q9NRF9
- Pathways
- DNA Damage Repair
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