NDC80 antibody (pSer55)

Catalog No. ABIN7468645

Product Details anti-NDC80 Antibody

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Binding Specificity: pSer55

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Kangaroo rat (Dipodomys sp.), Mouse, Rat

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser55 of human Hec1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Recommended Starting Dilutions:
For WB: 1:500-1:3000
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Comment:

Positive Control: HeLa , Blocking peptide (GTX70017-PEP) , HeLa (100 ng/ml Nocodazole treatment for 24 hr) Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.26 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: NDC80 kinetochore complex component (NDC80 Products)

Background:

Synonyms: NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80

Background: HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]

Molecular Weight: 80 kDa

Gene ID: 10403

UniProt: O14777

Pathways: Maintenance of Protein Location