DPF1 antibody

Catalog No. ABIN7468200

Product Details anti-DPF1 Antibody

Target: DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPF1 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DPF1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: IMR32 , U87-MG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.85 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DPF1

Target: DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))

Alternative Name: double PHD fingers 1 (DPF1 Products)

Synonyms: zgc:110104 antibody, DPF1 antibody, req antibody, xReq antibody, neud4 antibody, requiem antibody, neuro-d4 antibody, BAF45b antibody, NEUD4 antibody, Neud4 antibody, D4, zinc and double PHD fingers family 1 antibody, double PHD fingers 1 antibody, dpf1 antibody, DPF1 antibody, Dpf1 antibody

Background:

Synonyms: double PHD fingers 1 , BAF45b , NEUD4 , neuro-d4

Background: May have an important role in developing neurons by participating in regulation of cell survival, possibly as a neurospecific transcription factor. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.

Molecular Weight: 43 kDa

Gene ID: 8193

UniProt: Q92782