ETF1 antibody (N-Term)

Catalog No. ABIN7467264

Product Details anti-ETF1 Antibody

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ETF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human eRF1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.8 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ETF1

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Alternative Name: eukaryotic translation termination factor 1 (ETF1 Products)

Synonyms: D5S1995 antibody, ERF antibody, ERF1 antibody, RF1 antibody, SUP45L1 antibody, TB3-1 antibody, AI463371 antibody, D6Ertd109e antibody, eRF1 antibody, zeh0057 antibody, hm:zeh0057 antibody, wu:fa19e07 antibody, wu:fi31f01 antibody, cl1 antibody, RRF1 antibody, DDBDRAFT_0188023 antibody, DDBDRAFT_0191343 antibody, DDB_0188023 antibody, DDB_0191343 antibody, CG5605 antibody, Dm0184 antibody, Dmel\\CG5605 antibody, Group J antibody, group J antibody, l(3)00103 antibody, l(3)05637 antibody, l(3)77ABb antibody, l(3)neo28 antibody, Eukaryotic release factor 1 antibody, eukaryotic translation termination factor 1 antibody, eukaryotic translation termination factor 1b antibody, eukaryotic translation termination factor 1 S homeolog antibody, eukaryotic peptide chain release factor subunit 1 antibody, eukaryotic petide chain release factor subunit 1 antibody, eukaryotic release factor 1 antibody, Eukaryotic peptide chain release factor subunit 1 antibody, ETF1 antibody, Etf1 antibody, etf1b antibody, etf1 antibody, etf1.S antibody, Tb11.22.0012 antibody, THAPSDRAFT_22240 antibody, TTHERM_00292170 antibody, LOC692937 antibody, erf1 antibody, eRF1 antibody, erfa-1 antibody

Background: Eukaryotic translation termination factor 1 , D5S1995 , ERF , ERF1 , RF1 , SUP45L1 , TB3-1,Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]

Molecular Weight: 49 kDa

Gene ID: 2107

UniProt: P62495