UBE3C antibody

Catalog No. ABIN7466789

Product Details anti-UBE3C Antibody

Target: UBE3C (Ubiquitin Protein Ligase E3C (UBE3C))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE3C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human UBE3C. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for UBE3C

Target: UBE3C (Ubiquitin Protein Ligase E3C (UBE3C))

Alternative Name: ubiquitin protein ligase E3C (UBE3C Products)

Synonyms: UBE3C antibody, RGD1559986 antibody, zgc:63649 antibody, AI853514 antibody, mKIAA0010 antibody, HECTH2 antibody, kiaa0010 antibody, MGC146390 antibody, ubiquitin protein ligase E3C antibody, ubiquitin protein ligase E3C S homeolog antibody, UBE3C antibody, Ube3c antibody, ube3c antibody, ube3c.S antibody

Background: Ubiquitin protein ligase E3C , HECTH2,E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.

Molecular Weight: 124 kDa

Gene ID: 9690

UniProt: Q15386