RFWD3 antibody (C-Term)

Catalog No. ABIN7466618

Product Details anti-RFWD3 Antibody

Target: RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFWD3 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human RFWD3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MCF-7

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.96 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for RFWD3

Target: RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))

Alternative Name: ring finger and WD repeat domain 3 (RFWD3 Products)

Synonyms: RGD1305243 antibody, RFWD3 antibody, RNF201 antibody, BC027246 antibody, ring finger and WD repeat domain 3 antibody, Rfwd3 antibody, RFWD3 antibody

Background: Ring finger and WD repeat domain 3 , FANCW , RNF201,E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.

Molecular Weight: 85 kDa

Gene ID: 55159

UniProt: Q6PCD5