BCKDHA antibody (C-Term)
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- Target See all BCKDHA Antibodies
- BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This BCKDHA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Cross-Reactivity
- Human
- Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the C-terminus region of human BCKDHA. The exact sequence is proprietary.
- Isotype
- IgG
- Top Product
- Discover our top product BCKDHA Primary Antibody
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- Application Notes
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: HepG2
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))
- Alternative Name
- branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA Products)
- Synonyms
- wu:fd20d04 antibody, zgc:110049 antibody, BCKDE1A antibody, MSU antibody, MSUD1 antibody, OVD1A antibody, BCKDA antibody, E1a antibody, 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody, branched chain keto acid dehydrogenase E1, alpha polypeptide antibody, 2-oxoisovalerate dehydrogenase alpha subunit (branched-chain alpha-keto acid dehydrogenase E1 component alpha chain) (BCKDH E1-alpha) (BCKDE1A) antibody, branched chain ketoacid dehydrogenase E1, alpha polypeptide antibody, LOC704978 antibody, bckdha antibody, BCKDHA antibody, BckdhA antibody, Bckdha antibody
- Background
- Branched chain keto acid dehydrogenase E1, alpha polypeptide , BCKDE1A , MSU , MSUD1 , OVD1A,The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD, EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998 [PubMed 9582350]). The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2, MIM 248610), and a homodimeric dihydrolipoamide dehydrogenase (E3, MIM 238331). E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene (MIM 248611) encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.[supplied by OMIM]
- Molecular Weight
- 50 kDa
- Gene ID
- 593
- UniProt
- P12694
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