MED13 antibody

Catalog No. ABIN7464200

Product Details anti-MED13 Antibody

Target: MED13 (Mediator Complex Subunit 13 (MED13))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED13 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Knockdown/Knockout validation was supported by references (PMID:36220895)

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MED13. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MED13

Target: MED13 (Mediator Complex Subunit 13 (MED13))

Alternative Name: mediator complex subunit 13 (MED13 Products)

Synonyms: ARC250 antibody, DRIP250 antibody, HSPC221 antibody, THRAP1 antibody, TRAP240 antibody, 1110067M05Rik antibody, D030023K18 antibody, Thrap1 antibody, Trap240 antibody, med13 antibody, wu:fb66c06 antibody, wu:fc43h07 antibody, wu:fc43h08 antibody, zgc:153454 antibody, skuld-like antibody, mediator complex subunit 13 antibody, mediator complex subunit 13b antibody, MED13 antibody, Med13 antibody, med13b antibody

Background: Mediator complex subunit 13 , ARC250 , DRIP250 , HSPC221 , THRAP1 , TRAP240,This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator. [provided by RefSeq]

Molecular Weight: 239 kDa

Gene ID: 9969

UniProt: Q9UHV7

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha