RNA Polymerase II antibody (pSer5)
Reactivity: Human, Mouse
WB, IP, IHC (fp)
Host: Rabbit
Polyclonal
unconjugated
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- Target See all RNA Polymerase II products
- RNA Polymerase II
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Binding Specificity
- pSer5
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RNA Polymerase II antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
- Purpose
- Rabbit anti-RNA Polymerase II, Phospho (S5) Antibody, Affinity Purified
- Predicted Reactivity
- D. melanogaster
- Purification
- Affinity Purified
- Immunogen
- surrounding serine 5
- Isotype
- IgG
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- Application Notes
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IHC: 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IP: 2 - 10 μg/mg lysate
WB: 1:2,000 - 1:10,000
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Expiry Date
- 12 months
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- Target
- RNA Polymerase II
- Abstract
- RNA Polymerase II Products
- Synonyms
- DNA-directed RNA polymerase II subunit RPB7 antibody, RNA polymerase II antibody
- Background
- Background: RNA polymerase II is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RNA polymerase II subunit B1 (RPB1) is the largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit [taken from NCBI Entrez Gene (Gene ID: 5430)].
- Gene ID
- 5430
- NCBI Accession
- NP_000928
- UniProt
- P24928
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