SENP3 antibody (AA 275-325)

Catalog No. ABIN7454900

Product Details anti-SENP3 Antibody

Target: SENP3 (SUMO/sentrin Specific Peptidase 3 (SENP3))

Binding Specificity: AA 275-325

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SENP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-SENP3 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 275 and 325

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SENP3

Target: SENP3 (SUMO/sentrin Specific Peptidase 3 (SENP3))

Alternative Name: SENP3 (SENP3 Products)

Background: Background: Sentrin-specific protease 3 (SENP3) functions as a protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates.SENP3 deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability, and also deconjugates SUMO2 and SUMO3 from CDCA8. SENP3 can function as a redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. SENP3 is required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1 [taken from the Universal Protein Resource (UniProt) Q9H4L4].

Gene ID: 26168

NCBI Accession: NP_056485

UniProt: Q9H4L4