WHSC2 antibody (AA 499-549)

Catalog No. ABIN7454795

Product Details anti-WHSC2 Antibody

Target: WHSC2 (Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2))

Binding Specificity: AA 499-549

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WHSC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-NELFA Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: between AA 499 and 549

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for WHSC2

Target: WHSC2 (Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2))

Alternative Name: NELFA (WHSC2 Products)

Background: Background: NELFA (negative elongation factor A) is one of four subunits that make up the NELF transcription elongation factor. The four subunits of NELF are NELF A (WHSC2), NELFB, either NELFC or NELFD, and NELFE. NELF, along with DRB sensitivity-inducing factor (DSIF), functions to cause transcriptional pausing of RNA polymerase II. NELFA may play a role in Wolf-Hirschhorn syndrome (WHS).

Gene ID: 7469

UniProt: Q9H3P2

Pathways: Chromatin Binding