PUS1 antibody (AA 377-427)

Catalog No. ABIN7454768

Product Details anti-PUS1 Antibody

Target: PUS1 (Pseudouridylate Synthase 1 (PUS1))

Binding Specificity: AA 377-427

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-PUS1 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 377 and 427

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for PUS1

Target: PUS1 (Pseudouridylate Synthase 1 (PUS1))

Alternative Name: PUS1 (PUS1 Products)

Synonyms: PUS1 antibody, A730013B20Rik antibody, MPUS1 antibody, mPus1p antibody, MLASA1 antibody, pseudouridylate synthase 1 antibody, pseudouridylate synthase 1, putative antibody, pseudouridine synthase 1 antibody, PUS1 antibody, PVX_093530 antibody, PKH_011400 antibody, Pus1 antibody

Background: Background: Pus1 (pseudouridylate synthase 1) is an enzyme that converts uridine to pseudouridine in non-coding RNA substrates. Pus1 modifies uridines at specific positions in a subset of tRNAs. Pseudouridylation is known to affect the structure of tRNAs and stabilize base-stacking and base-pairing in the anticodon loop. Pus1 activity is important for proper folding and function of tRNAs. Pus1 has also been shown to pseudouridylate SRA (steroid receptor RNA activator) and function as a coactivator. Defects in pseudouridylation are associated with the human disease, MLASA (myopathy with lactic acidosis and sideroblastic anemia), an autosomal recessive oxidative phosphorylataion disorder specific to skeletal muscle and bone marrow.

Gene ID: 80324

NCBI Accession: NP_079491

UniProt: Q9Y606