CENPF antibody (AA 2350-2400)

Catalog No. ABIN7454765

Product Details anti-CENPF Antibody

Target: CENPF (Centromere Protein F (CENPF))

Binding Specificity: AA 2350-2400

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPF antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-CENP-F/Mitosin Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 2350 and 2400

Isotype: IgG

Application Details

Application Notes:

IHC: 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CENPF

Target: CENPF (Centromere Protein F (CENPF))

Alternative Name: CENP-F/Mitosin (CENPF Products)

Synonyms: LOC100222985 antibody, 6530404A22Rik antibody, AI325968 antibody, CENF antibody, Lek1 antibody, PRO1779 antibody, mitosin antibody, im:7140452 antibody, im:7144238 antibody, im:7154719 antibody, im:7156761 antibody, si:ch211-152h14.2 antibody, hcp-1 antibody, CMF1 antibody, centromere protein F antibody, CENPF antibody, Cenpf antibody, cenpf antibody

Background: Background: Centromere protein F (CENP-F) is a microtubule binding protein and component of the kinetochore. It is essential for kinetochore attachment, chromosome alignment, and the spindle checkpoint. CENP-F appears to be important to the association of the NudE-related proteins Ndel1 and Nde1 to the Lis1/dynein motor complex and their role in kinetochore function. CENP-F is a farnesylated protein and the farnesylation of CENP-F appears to be central to its role in mediating kinetochore-microtubule interactions as well as its degradation after mitosis. Recently, CENP-F expression has been correlated with markers of poor outcome in breast cancer.

Gene ID: 1063

UniProt: P49454

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection