AKAP8L antibody (AA 596-646)

Catalog No. ABIN7454742

Product Details anti-AKAP8L Antibody

Target: AKAP8L (A Kinase (PRKA) Anchor Protein 8-Like (AKAP8L))

Binding Specificity: AA 596-646

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP8L antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-AKAP8L/HA95 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 596 and 646

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for AKAP8L

Target: AKAP8L (A Kinase (PRKA) Anchor Protein 8-Like (AKAP8L))

Alternative Name: AKAP8L/HA95 (AKAP8L Products)

Synonyms: HA95 antibody, HAP95 antibody, NAKAP antibody, NAKAP95 antibody, Nakap95 antibody, zgc:85966 antibody, AKAP8L antibody, A-kinase anchoring protein 8 like antibody, A kinase (PRKA) anchor protein 8-like antibody, A-kinase anchoring protein 8 like L homeolog antibody, AKAP8L antibody, Akap8l antibody, akap8l antibody, akap8l.L antibody

Background: Background: AKAP8L/HA95 is a PKA anchoring protein that is localized to the nuclear envelope and associates with chromatin upon nuclear envelope breakdown during mitosis. AKAP8L/HA95 has been found to play an important role in the initiation of DNA replication and may function as a scaffold for proteins such as MCM2 and LAP2beta. Additionally, AKAP8L/HA95 has been shown to co-locate with the PKA C subunit in splicing factor compartments and is involved in the regulation of pre-mRNA splicing. Further support for a scaffolding and splicing role in the nucleus comes from the observation that AKAP8L/HA95 colocalizes with HypA, a splicing-like factor, and through this association may function as a docking site for the nuclear accumulation of Huntington protein as seen in Huntington's disease.

Gene ID: 26993

NCBI Accession: NP_055186

UniProt: Q9ULX6

Pathways: SARS-CoV-2 Protein Interactome