CDC42EP4 antibody (AA 250-300)

Catalog No. ABIN7454447

Product Details anti-CDC42EP4 Antibody

Target: CDC42EP4 (CDC42 Effector Protein (Rho GTPase Binding) 4 (CDC42EP4))

Binding Specificity: AA 250-300

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC42EP4 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-BORG4 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 250 and 300

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 5 - 10 μg/mg lysate

WB: Not recommended. A302-380A has not performed satisfactorily when used for WB of BORG4 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of BORG4.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CDC42EP4

Target: CDC42EP4 (CDC42 Effector Protein (Rho GTPase Binding) 4 (CDC42EP4))

Alternative Name: BORG4 (CDC42EP4 Products)

Synonyms: cdc42ep4 antibody, zgc:91889 antibody, wu:fb55a05 antibody, cep4 antibody, borg4 antibody, kaia1777 antibody, BORG4 antibody, CEP4 antibody, KAIA1777 antibody, 1500041M20Rik antibody, Borg4 antibody, CDC42 effector protein 4 antibody, CDC42 effector protein (Rho GTPase binding) 4a antibody, CDC42 effector protein 4 L homeolog antibody, CDC42 effector protein (Rho GTPase binding) 4 antibody, CDC42EP4 antibody, cdc42ep4a antibody, cdc42ep4 antibody, cdc42ep4.L antibody, Cdc42ep4 antibody

Background: Background: BORG4 (binder of Rho GTPases 4) is part of a family of proteins that function as negative regulators of CDC42 Rho GTPase signaling. The Borg family of proteins are also known as CEPs (Cdc effector proteins) and have been shown to act downstream of Cdc42 to influence the assembly of actin filaments and changes in cell shape.

Gene ID: 23580

NCBI Accession: NP_036253

UniProt: Q9H3Q1