Symplekin antibody (AA 200-250)

Catalog No. ABIN7454405

Product Details anti-Symplekin Antibody

Target: Symplekin (SYMPK)

Binding Specificity: AA 200-250

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Symplekin antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-Symplekin Antibody, Affinity Purified

Predicted Reactivity: Mouse

Purification: Affinity Purified

Immunogen: between AA 200 and 250

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended. Use rabbit anti-Symplekin antibody A301-464A or A301-465A.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for Symplekin

Target: Symplekin (SYMPK)

Alternative Name: Symplekin (SYMPK Products)

Background: Background: Symplekin is a component of tight junctions that also localizes to the nucleus and is a component of 3'-end processing machinery. As part of the mRNA 3'-end processing machinery symplekin is necessary for poly(A) tail elongation. Although histone mRNA 3'ends are not polyadenylated, symplekin has been shown to also play a role in 3'end maturation of histone mRNAs.

Gene ID: 8189

NCBI Accession: NP_004810

UniProt: Q92797