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MAD2 antibody (AA 100-150)

Reactivity: Human IP, IHC (fp) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7453589
  • Target See all MAD2 products
    MAD2
    Binding Specificity
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 100-150
    Reactivity
    • 7
    • 2
    • 1
    • 1
    Human
    Host
    • 7
    Rabbit
    Clonality
    • 7
    Polyclonal
    Conjugate
    • 7
    This MAD2 antibody is un-conjugated
    Application
    • 6
    • 2
    • 2
    • 1
    • 1
    Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
    Purpose
    Rabbit anti-MAD2 Antibody, Affinity Purified
    Purification
    Affinity Purified
    Immunogen
    between AA 100 and 150
    Isotype
    IgG
  • Application Notes

    IHC: 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

    IP: 2 - 10 μg/mg lysate

    WB: Not recommended. Use rabbit anti-MAD2 antibody ABIN7450431.

    Restrictions
    For Research Use only
  • Concentration
    1000 μg/mL
    Buffer
    Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Expiry Date
    12 months
  • Target
    MAD2
    Abstract
    MAD2 Products
    Synonyms
    AA673185 antibody, MAD2 antibody, MXD2 antibody, MXI antibody, bHLHc11 antibody, MAD2 mitotic arrest deficient-like 1 antibody, MAD2 mitotic arrest deficient-like 1 (yeast) antibody, MAX interactor 1, dimerization protein antibody, Mad2l1 antibody, MAD2L1 antibody, MXI1 antibody
    Background
    Background: MAD2 (Mitotic Arrest Deficiency 2) is a component of the mitotic spindle-assembly checkpoint that ensures that all chromosomes are aligned at the metaphase plate before the onset of anaphase. MAD2 is a subunit of the MCC complex that includes BubR1, Bub3, Cdc20 and Mad2, which may function to inhibit the APC/c (Anaphase Promoting Complex/Cyclosome). MAD2 is regulated by MAD1.
    Gene ID
    4085
    NCBI Accession
    NP_002349
    UniProt
    Q13257
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