RBM17 antibody (AA 25-75)

Catalog No. ABIN7453552

Product Details anti-RBM17 Antibody

Target: RBM17 (RNA Binding Motif Protein 17 (RBM17))

Binding Specificity: AA 25-75

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBM17 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-SPF45 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 25 and 75

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended. Use rabbit anti-SPF45 antibody ABIN7453790.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for RBM17

Target: RBM17 (RNA Binding Motif Protein 17 (RBM17))

Alternative Name: SPF45 (RBM17 Products)

Synonyms: zgc:55840 antibody, SPF45 antibody, 2700027J02Rik antibody, RNA binding motif protein 17 antibody, RNA binding motif protein 17 L homeolog antibody, Rbm17 antibody, rbm17 antibody, RBM17 antibody, rbm17.L antibody

Background: Background: Splicing factor 45 (SPF45) is an RNA splicing factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. SPF45 is involved in the regulation of alternative splicing and the utilization of cryptic splice sites. It has been shown to promote the utilization of a cryptic splice site created by the beta-110 mutation in the human beta-thalassemia gene. The resulting frameshift leads to sickle cell anemia [taken from the Universal Protein Resource (UniProt) Q96125].

Gene ID: 84991

NCBI Accession: NP_116294

UniProt: Q96I25