POLE3 antibody (AA 97-147)

Catalog No. ABIN7453486

Product Details anti-POLE3 Antibody

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Binding Specificity: AA 97-147

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLE3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-PolE3/p17 Antibody, Affinity Purified

Predicted Reactivity: Rat,Bovine,Orangutan

Purification: Affinity Purified

Immunogen: between AA 97 and 147

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for POLE3

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Alternative Name: PolE3/p17 (POLE3 Products)

Synonyms: NCU04548.1 antibody, GB14966 antibody, zgc:73351 antibody, 1810034K18Rik antibody, YBL1 antibody, CHARAC17 antibody, CHRAC17 antibody, p17 antibody, DNA polymerase epsilon 3, accessory subunit antibody, polymerase (DNA directed), epsilon 3, accessory subunit antibody, DNA polymerase epsilon antibody, DNA polymerase epsilon p17 subunit antibody, DNA polymerase epsilon subunit 3 antibody, polymerase (DNA directed), epsilon 3 (p17 subunit) antibody, POLE3 antibody, pole3 antibody, NCU04548 antibody, CpipJ_CPIJ008666 antibody, Bm1_04865 antibody, Bm1_07285 antibody, CTRG_00708 antibody, LOAG_09571 antibody, LOC726923 antibody, Pole3 antibody

Background: Background: PolE3/p17 is a subunit of DNA polymerase epsilon that plays a role in DNA replication and repair. PolE3/p17 contains histone-fold motifs which play a role in protein-protein and protein-DNA interactions. PolE3/p17 interacts with CHRAC1 (chromatin assembly complex 1), ACF1, and ISWI/SNF2H proteins to form the CHRAC ISWI chromatin-remodeling complex.

Gene ID: 54107

NCBI Accession: NP_059139

UniProt: Q9NRF9

Pathways: DNA Damage Repair