CAND1 antibody (AA 1180-1230)

Catalog No. ABIN7453479

Product Details anti-CAND1 Antibody

Target: CAND1 (Cullin-Associated and Neddylation-Dissociated 1 (CAND1))

Binding Specificity: AA 1180-1230

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAND1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-CAND1 Antibody, Affinity Purified

Predicted Reactivity: Rat,Bovine,Orangutan

Purification: Affinity Purified

Immunogen: between AA 1180 and 1230

Isotype: IgG

Application Details

Application Notes:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for CAND1

Target: CAND1 (Cullin-Associated and Neddylation-Dissociated 1 (CAND1))

Alternative Name: CAND1 (CAND1 Products)

Background: Background: Cullin-associated NEDD8-dissociated protein 1 (CAND1) is a regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. CAND1 prevents neddylation of CUL1 by physically blocking access to the neddylation site and disrupts interactions between CUL1 and SKP1A and between CUL1 and F-box proteins. CAND1 also binds TATA binding protein and enhances transcription from various promoters [taken from the Universal Protein Resource (UniProt) Q86VP6].

Gene ID: 55832

NCBI Accession: NP_060918

UniProt: Q86VP6

Pathways: Regulation of Actin Filament Polymerization