PCNT antibody (AA 175-225)

Catalog No. ABIN7453380

Product Details anti-PCNT Antibody

Target: PCNT (Pericentrin (PCNT))

Binding Specificity: AA 175-225

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNT antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Purpose: Rabbit anti-Pericentrin/Kendrin Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 175 and 225

Isotype: IgG

Application Details

Application Notes:

ICC-IF: 1:1,000 - 1:5,000. Formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for PCNT

Target: PCNT (Pericentrin (PCNT))

Alternative Name: Pericentrin/Kendrin (PCNT Products)

Background: Background: Pericentrin (PCNT) is a large centrosomal protein that binds calmodulin and mediates assembly of the mitotic spindle apparatus. Depletion of PCNT has been shown to cause apoptosis and activation of the G2/M checkpoint. Recently, mutations in the pericentrin gene have been identified as the cause of primordial dwarfism type II (MOPDII) and Seckel syndrome. Additionally, in cells of Seckel syndrome patients, PCNT has been found to be important to the DNA damage response and ATR-dependent checkpoint signaling.

Gene ID: 5116

NCBI Accession: NP_006022

UniProt: O95613

Pathways: Sensory Perception of Sound, M Phase, SARS-CoV-2 Protein Interactome