FAM105B antibody (AA 75-125)

Catalog No. ABIN7452665

Product Details anti-FAM105B Antibody

Target: FAM105B (Family with Sequence Similarity 105, Member B (FAM105B))

Binding Specificity: AA 75-125

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAM105B antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Purpose: Rabbit anti-OTULIN Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 75 and 125

Isotype: IgG

Application Details

Application Notes:

IP: 20-40 μL/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for FAM105B

Target: FAM105B (Family with Sequence Similarity 105, Member B (FAM105B))

Alternative Name: OTULIN (FAM105B Products)

Synonyms: C79097 antibody, OTU deubiquitinase with linear linkage specificity antibody, OTULIN antibody, Otulin antibody

Background: Background: Otulin is a deubiquitinase that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. Required during angiogenesis, craniofacial and neuronal development by regulating the canonical Wnt signaling together with the LUBAC complex. Acts as a negative regulator of NF-kappa-B by regulating the activity of the LUBAC complex. OTULIN function is mainly restricted to homeostasis of the LUBAC complex: acts by removing 'Met-1'-linked autoubiquitination of the LUBAC complex, thereby preventing inactivation of the LUBAC complex. Acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. In myeloid cell, required to prevent unwarranted secretion of cytokines leading to inflammation and autoimmunity by restricting linear polyubiquitin formation. Plays a role in innate immune response by restricting linear polyubiquitin formation on LUBAC complex in response to NOD2 stimulation, probably to limit NOD2-dependent proinflammatory signaling. [taken from the Universal Protein Resource (UniProt) Q96BN8].

Gene ID: 90268

UniProt: Q96BN8