SPEN antibody (AA 3375-3425)

Catalog No. ABIN7452220

Product Details anti-SPEN Antibody

Target: SPEN (Spen Homolog, Transcriptional Regulator (SPEN))

Binding Specificity: AA 3375-3425

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPEN antibody is un-conjugated

Application: Immunoprecipitation (IP)

Purpose: Rabbit anti-SHARP Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: between AA 3375 and 3425

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: Not recommended. ABIN7451040 can be used successfully for WB of enriched preparations (e.g. immunoprecipitated) SHARP.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for SPEN

Target: SPEN (Spen Homolog, Transcriptional Regulator (SPEN))

Alternative Name: SHARP (SPEN Products)

Background: Background: SHARP functions as a transcriptional repressor by interacting with proteins involved in deacetylation such as the NuRD (nucleosome remodeling) complex. SHARP can also repress transcription by interacting with corepressors such as SMRT/NCoR (silencing mediator for retinoid and thyroid receptors/nuclear receptor corepressor). SHARP is able to suppress transcription through the Notch/RBP-J signaling pathway by competing RBP-J binding with the intracellular domain of Notch.

Gene ID: 23013

NCBI Accession: NP_055816

UniProt: Q96T58