NSMCE2 antibody (AA 197-247)

Catalog No. ABIN7451060

Product Details anti-NSMCE2 Antibody

Target: NSMCE2 (E3 SUMO-Protein Ligase NSE2 (NSMCE2))

Binding Specificity: AA 197-247

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSMCE2 antibody is un-conjugated

Application: Immunoprecipitation (IP)

Purpose: Rabbit anti-MMS21 Antibody, Affinity Purified

Purification: Affinity Purified

Immunogen: Between AA 197 and 247

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 10 μg/mg lysate

WB: Not recommended. ABIN7451060 has not performed satisfactorily when used for WB of MMS21 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of MMS21.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for NSMCE2

Target: NSMCE2 (E3 SUMO-Protein Ligase NSE2 (NSMCE2))

Alternative Name: MMS21 (NSMCE2 Products)

Synonyms: 1110014D18Rik antibody, AI661537 antibody, RGD1305156 antibody, C8orf36 antibody, MMS21 antibody, NSE2 antibody, ZMIZ7 antibody, Non-SMC element 2 antibody, NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase antibody, NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase L homeolog antibody, Smc5-6 complex non-SMC subunit 2 antibody, Nsmce2 antibody, NSMCE2 antibody, nsmce2.L antibody, nse2 antibody

Background: Background: The human MMS21 (Methyl Methanesulfonate-Sensitivity protein 21) homolog is also known as E3 SUMO-protein ligase NSE2. MMS21 is an E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks [taken from the Universal Protein Resource (UniProt) Q96MF7].

Gene ID: 286053

NCBI Accession: NP_775956

UniProt: Q96MF7