tdTomato antibody (DyLight 550)

Catalog No. ABIN7273113

Product Details anti-tdTomato Antibody (DyLight 550)

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Reactivity: Discosoma

Host: Goat

Clonality: Polyclonal

Conjugate: This tdTomato antibody is conjugated to DyLight 550

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Cross-Reactivity (Details): Reacts against tdTomato, mCherry, red fluorescent protein (DsRed) from so-called disc corals of Discosoma species.

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide produced in E. coli

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000, IF:1:50-1:500, IHC-F:1:50-1:1,000

Comment:

In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).

Restrictions: For Research Use only

Handling

Concentration: 2.5 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.

Target Details for tdTomato

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Alternative Name: tdTomato (tdTomato Products)

Background: TdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.