tdTomato antibody

Catalog No. ABIN7273105

Product Details anti-tdTomato Antibody

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Reactivity: Discosoma

Host: Goat

Clonality: Polyclonal

Conjugate: This tdTomato antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity (Details): Reacts against tdTomato, mCherry, RFP

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Comment:

In 293HEK cells transfected with cds plasmid detects a band of 54 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).

Restrictions: For Research Use only

Handling

Concentration: 2 mg/mL

Buffer: NaHCO3 100 mM pH 9, no preservatives

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.

Target Details for tdTomato

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Alternative Name: tdTomato (tdTomato Products)

Background: TdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.