Recombinant mRuby antibody (Biotin)
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- Target
- mRuby
- Antibody Type
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
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Reactivity
- Entacmaea quadricolor
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Host
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Alpaca
- Expression System
- E.coli
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Clonality
- Monoclonal
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Conjugate
- Biotin
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Application
- Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Recognizes most common mRuby derivatives
- Purification
- Produced in: E.coli
- Immunogen
- sdAb anti-mRUBY is derived from an in-house developed single-domain antibody (sdAb) that recognizes mRuby and mRuby derivatives, which is a bright monomeric red fluorescent protein derived from Entacmaea quadricolor.
- Clone
- 1F8
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anti-mRuby antibody (Atto 488)
Reactivity: Entacmaea quadricolor IF, ICC, FACS Host: Alpaca Monoclonal 1F8 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
anti-mRuby antibodyReactivity: Discosoma IF, IEM, IHC (fro), IHC (p), WB Host: Goat Polyclonal unconjugated
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- Application Notes
- Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconsitute with 250 µL 50% glycerol for storage at -80 °C
- Buffer
- sdAbs are lyophilized from PBS pH 7.4.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
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- Target
- mRuby
- Background
- MRuby is a bright monomeric red fluorescent protein, which has more than 20 mutations compared to its wild-type predecessor (eqFP611) found in Entacmaea quadricolor . mRuby, mRuby2 & mRuby3 are all exited at 560 nm, with mRuby3 being the brightest.
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