Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconsitute with 250 µL pure water for immidate use
Buffer
sdAbs are lyohilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA pH 6.
Storage
4 °C/-20 °C
Storage Comment
Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
A breakthrough in the field of biology and bioluminescence started in the 60s with the discovery of a glowing protein obtained from the jellyfish Aequorea victoria by Osamu Shimomura and colleagues. However, it was only in the early 90s when the green fluorescent protein (GFP) sequence was cloned and used inside a foreign organism as a fluorescent marker. Today more than 800 entries of various fluorescent proteins can be found in an open-source database with the most currently available variants here.