METTL16 antibody (AA 263-562)

Catalog No. ABIN7268501

Product Details anti-METTL16 Antibody

Target: METTL16 (Methyltransferase Like 16 (METTL16))

Binding Specificity: AA 263-562

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This METTL16 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Purpose: METTL16 Rabbit pAb

Sequence: RIQGVPKVTY TEFCQGRTMR WALAWSFYDD VTVPSPPSKR RKLEKPRKPI TFVVLASVMK ELSLKASPLR SETAEGIVVV TTWIEKILTD LKVQHKRVPC GKEEVSLFLT AIENSWIHLR RKKRERVRQL REVPRAPEDV IQALEEKKPT PKESGNSQEL ARGPQERTPC GPALREGEAA AVEGPCPSQE SLSQEENPEP TEDERSEEKG GVEVLESCQG SSNGAQDQEA SEQFGSPVAE RGKRLPGVAG QYLFKCLINV KKEVDDALVE MHWVEGQNRD LMNQLCTYIR NQIFRLVAVN

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 263-562 of human METTL16 (NP_076991.3).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200,IP,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for METTL16

Target: METTL16 (Methyltransferase Like 16 (METTL16))

Alternative Name: METTL16 (METTL16 Products)

Synonyms: fi09d09 antibody, im:7148386 antibody, mett10d antibody, wu:fi09d09 antibody, zgc:100981 antibody, zgc:158576 antibody, METT10D antibody, 2610100D03Rik antibody, 2810013M15Rik antibody, A830095F14Rik antibody, AI846133 antibody, Mett10d antibody, methyltransferase like 16 antibody, methyltransferase like 16 L homeolog antibody, mettl16 antibody, mettl16.L antibody, METTL16 antibody, Mettl16 antibody

Background: RNA N6-methyltransferase that methylates adenosine residues at the N(6 position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts. Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs. In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure. Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, preventing recognition of their 3'-splice site by U2AF1/U2AF35, thereby inhibiting splicing and protein production of S-adenosylmethionine synthase. In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A. In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA: specifically N6-methylates adenine in position 43 of U6 snRNAs. Also able to bind various lncRNAs, such as 7SK snRNA (7SK RNA or 7SL RNA. Specifically binds the 3'-end of the MALAT1 long non-coding RNA.,METTL16,METT10D,Epigenetics & Nuclear Signaling,RNA Binding,Cell Biology & Developmental Biology,METTL16

Molecular Weight: 25kDa/63kDa

Gene ID: 79066

UniProt: Q86W50