ITCH antibody (AA 50-150)

Catalog No. ABIN7268033

Product Details anti-ITCH Antibody

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Binding Specificity: AA 50-150

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITCH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: [KO Validated] ITCH Rabbit pAb

Sequence: QSKKTEKCNN TNSPKWKQPL TVIVTPVSKL HFRVWSHQTL KSDVLLGTAA LDIYETLKSN NMKLEEVVVT LQLGGDKEPT ETIGDLSICL DGLQLESEVV T

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Grade: KO Validated

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ITCH (NP_001244066.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH (ITCH Products)

Background: This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.,ITCH,ADMFD,AIF4,AIP4,NAPP1,Signal Transduction,Cell Biology & Developmental Biology,Apoptosis,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,Notch Signaling Pathway,Immunology & Inflammation,NF-kB Signaling Pathway,ITCH

Molecular Weight: 103kDa

Gene ID: 83737

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events