DDDDK Tag antibody (Magnetic Agarose Beads)

Catalog No. ABIN7533455

Product Details anti-DDDDK Tag Antibody (Magnetic Agarose Beads)

Target: DDDDK Tag

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DDDDK Tag antibody is conjugated to Magnetic Agarose Beads

Application: Immunoprecipitation (IP)

Purpose: Magnetic Beads-conjugated Mouse anti DDDDK-Tag mAb

Sequence: DYKDDDDK

Characteristics: Tag Antibodies

Purification: Affinity purification

Immunogen: A synthetic peptide corresponding to DDDDK tag.

Isotype: IgG

Application Details

Application Notes: IP,1:50 - 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C. Avoid freeze / thaw cycles.

Target Details for DDDDK Tag

Target: DDDDK Tag

Alternative Name: DDDDK (DDDDK Tag Products)

Background: FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.,DDDDK,DDDDK tag,DDDDK-tag,DDDDK