AKT 1/2/3 antibody

Catalog No. ABIN7265520

Product Details anti-AKT 1/2/3 Antibody

Target: AKT 1/2/3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT 1/2/3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purpose: Pan-Akt Rabbit pAb

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: A synthetic peptide of human AKT1/AKT2/AKT3

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:1000,IHC,1:50 - 1:100,IF,1:50 - 1:100,IP,1:50 - 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for AKT 1/2/3

Target: AKT 1/2/3

Alternative Name: AKT1/AKT2/AKT3 (AKT 1/2/3 Products)

Background: Human AKT serine-threonine protein kinase family includes three members AKT1,AKT2, AKT3, which are also often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene.,AKT1/AKT2/AKT3,Protein phosphorylation,AKT1/AKT2/AKT3

Molecular Weight: 48kDa/55kDa/51kDa/54kDa

Gene ID: 207, 208, 10000

UniProt: P31749, P31751, Q9Y243