PCLO antibody

Catalog No. ABIN7233031

Product Details anti-PCLO Antibody

Target: PCLO (Piccolo (Presynaptic Cytomatrix Protein) (PCLO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCLO antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Anti-Piccolo Mouse Monoclonal Antibody

Specificity: This antibody recognizes human and rat Piccolo isoforms (other species not yet tested). It does not cross-react with Bassoon.

Cross-Reactivity: Human, Rat

Immunogen: Full-length human Piccolo protein (accession no. BC001304.1)

Clone: 6H9-B6

Isotype: IgG2b

Application Details

Application Notes: Immunoblotting: use at 1-10 μg/mL. A band of ~550 kDa is detected
ELISA: use at 1-10 μg/mL with Piccolo on the solid phase.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Rat brain lysate.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

Target Details for PCLO

Target: PCLO (Piccolo (Presynaptic Cytomatrix Protein) (PCLO))

Alternative Name: Pclo (PCLO Products)

Synonyms: pclo antibody, wu:fj53e09 antibody, Acz antibody, Pico antibody, mKIAA0559 antibody, aczonin antibody, PCLO antibody, ACZ antibody, piccolo presynaptic cytomatrix protein antibody, piccolo presynaptic cytomatrix protein a antibody, piccolo (presynaptic cytomatrix protein) antibody, PCLO antibody, pcloa antibody, Pclo antibody, LOC768552 antibody

Background: Protein piccolo,Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:19812333). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (By similarity). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Organizes as well the readily releasable pool of synaptic vesicles and safeguards a fraction of them to be not immediately available for action potential-induced release (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy (PubMed:28231469) (By similarity). Mediates also synapse to nucleus communication leading to rUniProtKB:Q9JKS6, PubMed:19812333, PubMed:28231469}.,Piccolo is a novel component of the presynaptic cytoskeletal matrix (PCM) assembled at the active zone of neurotransmitter release. Piccolo is a multidomain zinc finger protein structurally related to Bassoon, another PCM protein. Both proteins share components of glutamatergic and GABAergic CNS synapses. Piccolo zinc fingers interact with the dual prenylated rab3A and VAMP2/Synaptobrevin II receptor PRA1. Piccolo is involved in endo- and exocytosis of synaptic vesicles and has been shown to interfere with clathrin- mediated endocytosis by binding to the F- actin and dynamin bind protein Abp1.,Cell junction, synapse, presynaptic active zone,Aczonin, Brain-derived HLMN protein, Multidomain presynaptic cytomatrix protein

UniProt: Q9QYX7

Pathways: Hormone Transport, Synaptic Vesicle Exocytosis