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Smad2/3 antibody

SMAD2/3 Reactivity: Human, Rat, Mouse, Monkey WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7217046
  • Target See all Smad2/3 (SMAD2/3) products
    Smad2/3 (SMAD2/3) (Smad2/3 Transcription Factor (SMAD2/3))
    Reactivity
    • 45
    • 33
    • 32
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    Human, Rat, Mouse, Monkey
    Host
    • 43
    • 2
    • 1
    Rabbit
    Clonality
    • 46
    Polyclonal
    Conjugate
    • 21
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Smad2/3 antibody is un-conjugated
    Application
    • 35
    • 14
    • 13
    • 13
    • 10
    • 7
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purpose
    Smad2/3 Polyclonal Antibody
    Specificity
    Smad2/3 Polyclonal Antibody detects endogenous levels of Smad2/3 protein.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
    Immunogen
    Synthesized peptide derived from human Smad2/3 around the non-phosphorylation site of T8
    Isotype
    IgG
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
    Comment

    Primary Antibody

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
  • Target
    Smad2/3 (SMAD2/3) (Smad2/3 Transcription Factor (SMAD2/3))
    Alternative Name
    Smad2/3 (SMAD2/3 Products)
    Background
    Rabbit Anti-Smad2/3 Polyclonal Antibody,SMAD2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, hSMAD2, SMAD3, MADH3, Mothers against decapentaplegic,Mothers against decapentaplegic homolog 2 encoded by SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF) -beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.,Mothers against decapentaplegic homolog 2
    Molecular Weight
    observerd band 48kDa
    Gene ID
    4087, 4088
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