RAD52 antibody

Catalog No. ABIN7216752

Product Details anti-RAD52 Antibody

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD52 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rad52 Polyclonal Antibody

Specificity: Rad52 Polyclonal Antibody detects endogenous levels of Rad52 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Rad52 around the non-phosphorylation site of Y104

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for RAD52

Target: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Alternative Name: Rad52 (RAD52 Products)

Synonyms: Rad52yh antibody, RAD52 antibody, zgc:112400 antibody, LOC100223085 antibody, MGC115453 antibody, RAD52 homolog, DNA repair protein antibody, RAD52 homolog (S. cerevisiae) antibody, RAD52 homolog, DNA repair protein S homeolog antibody, recombinase antibody, DNA repair and recombination protein, putative antibody, RAD52 protein antibody, RAD52 antibody, Rad52 antibody, rad52 antibody, rad52.S antibody

Background: Rabbit Anti-Rad52 Polyclonal Antibody,RAD52, DNA repair protein RAD52 homolog,RAD52 homolog, DNA repair protein encoded by RAD52 shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. RAD52 product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of RAD52 is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of RAD52 have been described, but their full-length nature is not known.,DNA repair protein RAD52 homolog

Molecular Weight: observerd band 46kDa

Gene ID: 5893

UniProt: P43351

Pathways: DNA Damage Repair